Cyclin B1 Antibody
P34 (Cdc2) Cyclin B1Cdk1
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 1-433) was used as the immunogen for this Cyclin B1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the Cyclin B1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Cyclin B1 antibody is available for research use only.
Storage Conditions
After reconstitution, the Cyclin B1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Cyclin B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear, cytoplasmic
Image Legend
Western blot testing of Cyclin B1 antibody and Lane 1: HeLa; 2: 293T; 3: MCF-7; 4: COLO320 lysate. Predicted molecular weight: 48-60 kDa.
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