Amyloid beta Antibody / APP

Amyloid beta, also called Abeta and APP, denotes peptides that are crucially involved in Alzheimers disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. Several potential activities have been discovered for Amyloid beta, including activation of kinase enzymes, functioning as a transcription factor, and anti-microbial activity (potentially associated with it pro-inflammatory activity) . Moreover, monomeric Amyloid beta is indicated to protect neurons by quenching metal-inducible oxygen radical generation and thereby inhibiting neurotoxicity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IF/ICC (FFPE) : 2-4 µg/mL

UniProt

P05067

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human APP (DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA) was used as the immunogen for this Amyloid beta antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Reconstitution

After reconstitution, the Amyloid beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Amyloid beta antibody is available for research use only.

Storage Conditions

After reconstitution, the Amyloid beta antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Amyloid beta antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, membrane

Image Legend

Western blot testing of human 1) HeLa, 2) U-87 MG, 3) T-47D, 4) A549, 5) U-2 OS, 6) rat brain and 7) mouse brain lysate with Amyloid beta antibody. Predicted molecular weight 79~120 kDa depending on glycosylation level.