Galectin 3 Antibody

Galectin 3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, it located on chromosome 14, locus q21-q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin 3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/10^6 cells

Host

Rabbit

Reactivity

Human

Immunogen

Human partial recombinant protein (AA 139-250) was used as the immunogen for this Galectin 3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Galectin 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Galectin 3 antibody is available for research use only.

Storage Conditions

After reconstitution, the Galectin 3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Galectin 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, cell surface

Image Legend

Western blot testing of human 1) HeLa, 2) SiHa, 3) U-87 MG, 4) RT4, 5) HaCaT, 6) PC-3 and 7) T-47D cell lysate with Galectin 3 antibody. Predicted molecular weight ~26 kDa.