P27KIP1 Antibody

Cyclin-dependent kinase inhibitor 1B, also known as KIP1 or P27, is an enzyme inhibitor that in humans is encoded by the CDKN1B gene. It encodes a protein which belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. p27KIP1 can inhibit both CDK activation and the kinase activity of assembled and activated cyclin-CDK. The function of the protein is associated with an aggressive phenotype in human breast cancer. Downregulation of p27KIP1 by CK2-alpha-prime is necessary for development of agonist- and stress-induced cardiac hypertrophy.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunocytochemistry (FFPE) : 2-4 µg/mL

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Human partial recombinant protein (AA 10-198) was used as the immunogen for this p27KIP1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the p27KIP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This p27KIP1 antibody is available for research use only.

Storage Conditions

After reconstitution, the p27KIP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the p27KIP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.