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CNTF Antibody

Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene. The protein encoded by this gene is a polypeptide hormone andnerve growth factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neural populations includingastrocytes. It is mapped to 11q12.1. In addition to neurotrophic effects on parasympathetic neurons, CNTF was shown to have activities on sympathetic and sensory neurons. It appears not to be involved in motor neuron survival during development and has a trophic influence on degenerating striatal neurons as well as on critical nonstriatal regions. CNTF plays a role in a cardiac signal transduction pathway that regulates obesity-related left ventricular hypertrophy.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (rat protein tested) ; request BSA free format for coating

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Rat partial recombinant protein (AA 2-200) was used as the immunogen for this CNTF antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ELISA (protein)

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

The lyophilized CNTF antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

Limitations

This CNTF antibody is available for research use only.

Storage Conditions

The lyophilized CNTF antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the CNTF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of CNTF antibody and rat samples 1: PC12; 2: NRK; 3: RH35; 4: brain; 5: liver; 6: spleen; 7: testis lysate. Expected/observed molecular weight ~23 kDa.

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