Src Antibody

Sarcoma is a proto-oncogenic tyrosine kinase originally discovered by J. Michael Bishop and Harold E. Varmus. It belongs to a family of non-receptor tyrosine kinases called Src family kinases. The human proto-oncogene was assigned to chromosome 20. Its gene is mapped to 20q12-q13. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry. This proto-oncogene may play a role in the regulation of embryonic development and cell growth.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P12931

Host

Rabbit

Reactivity

Human

Immunogen

Human partial recombinant protein (AA 2-368) was used as the immunogen for this Src antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Src antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Src antibody is available for research use only.

Storage Conditions

After reconstitution, the Src antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear, cell membrane

Image Legend

IHC staining of FFPE human rectal cancer tissue with Src antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.