MPO Antibody / Myeloperoxidase

Myeloperoxidase is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. MPO is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. Located in the nucleus as well as in the cytoplasm, intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL

Host

Rabbit

Reactivity

Human

Immunogen

Human partial recombinant protein (AA 406-745) was used as the immunogen for this MPO antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the MPO antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MPO antibody is available for research use only.

Storage Conditions

After reconstitution, the MPO antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the MPO antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear and cytoplasmic

Image Legend

Flow cytometry testing of human HL60 cells with MPO antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= MPO antibody.