IL6 Antibody
Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (mouse protein tested) ; request BSA free format for coating
Host
Rabbit
Reactivity
Mouse
Immunogen
Mouse partial recombinant protein (AA 25-211) was used as the immunogen for this IL6 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA (protein)
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
Limitations
This IL6 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Variations in secondary/substrate sensitivities and test protocols may require the IL6 antibody to be titrated for optimal performance.
Image Legend
Western blot testing of IL6 antibody and Lane 1: mouse HEPA1-6; 2: (m) NIH3T3 lysate. Expected molecular weight ~24 kDa.
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