Bid Antibody / BH3-Interacting Domain Death Agonist

Bid (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL (human), IHC (FFPE) : 0.5-1 µg/mL (human; mouse; rat), Immunocytochemistry: 0.5-1 µg/mL (human)

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Human partial recombinant protein (AA 1-195) was used as the immunogen for this Bid antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the Bid antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Bid antibody is available for research use only.

Storage Conditions

After reconstitution, the Bid antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Bid antibody may be required due to differences in protocols and secondary/substrate sensitivity.