ICAM-1 Antibody / CD54

Intercellular adhesion molecule-1 is a ligand for LFA-1, a beta 2 leukocyte integrin. In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer Leu-CAMa/LFA-1 and its ligand ICAM-1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemisty (FFPE) : 0.5-1 µg/mL, Immunofluorescence: 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells

Host

Rabbit

Reactivity

Human

Immunogen

Human partial recombinant protein (AA 214-532) was used as the immunogen for this ICAM-1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the ICAM-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ICAM-1 antibody is available for research use only.

Storage Conditions

After reconstitution, the ICAM-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ICAM-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of ICAM-1 antibody and Lane 1: HEPG2; 2: K562; 3: A549; 4: HT1080 lysate. Predicted molecular weight: ~58/75-115 kDa (unmodified/glycosylated) .