IL-18 Antibody

Interleukin-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL-18 is a cytokine that modulates both T helper type 1 (Th1) and Th2 responses. It is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL

Host

Rabbit

Reactivity

Rat, Mouse

Immunogen

Rat partial recombinant protein (AA 37-194) was used as the immunogen for this IL-18 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.005% sodium azide

Reconstitution

After reconstitution, the IL-18 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This IL-18 antibody is available for research use only.

Storage Conditions

After reconstitution, the IL-18 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the IL-18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.