Adiponectin Antibody

Adiponectin is a protein which in humans is encoded by the ADIPOQ gene. It is mapped to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, it has been showed that Adiponectin is expressed by differentiated adipocytes as a 30-kD protein. By sequence comparisons, it has been found that links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Rat partial recombinant protein (AA 19-244) was used as the immunogen for this Adiponectin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

The lyophilized Adiponectin antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

Limitations

This Adiponectin antibody is available for research use only.

Storage Conditions

The lyophilized Adiponectin antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the Adiponectin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Adiponectin antibody and rat samples 1: testis; 2: spleen; 3: skeletal muscle; 4: heart; 5: liver lysate. Routinely observed at 26~30kDa.