P53 Antibody
The p53 tumor antigen induces cell cycle arrest or apoptosis in response to sub-lethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P04637
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 74-393) was used as the immunogen for this p53 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
Limitations
This p53 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Titration of the p53 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
Frequently Asked Questions
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