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CADM1 Antibody

Cell adhesion molecule 1 is a protein that, in humans, is encoded by the CADM1 gene. This gene is also known as IGSF4, SYNCAM or TSLC1, and it is mapped to 11q23.3. It functions as a homophilic cell adhesion molecule at the synapse. Expression of the isolated cytoplasmic tail of CADM1 in neurons inhibited synapse assembly. Conversely, expression of full-length protein in non-neuronal cells induced synapse formation by cocultured hippocampal neurons with normal release properties. It has been shown to interact with EPB41L3.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q9BY67

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the N-terminus of human SynCAM/CADM1 (VIQLLNPNRQTIYFRDFRPL) was used as the immunogen for this CADM1 antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the CADM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CADM1 antibody is available for research use only.

Storage Conditions

After reconstitution, the CADM1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the CADM1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of CADM1 antibody and Lane 1: A549; 2: Jurkat; 3: Raji; 4: HeLa lysate. Predicted molecular weight: ~48/37kDa (isoforms 1/2) with N-linked and O-linked glycosylation each possibly adding ~25kDa to the observed size.

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