HNF1B Antibody

Hepatocyte nuclear factor 1 homeobox B, also known as Transcription factor 2 (TCF2), is a human gene. It is a member of the homeodomain-containing superfamily of transcription factors. The HNF1B protein is believed to form heterodimers with another liver-specific member of this transcription factor family, TCF1. It functions as both a classic transcriptional activator and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. HNF1B also can regulate renal tubulogenesis by controlling expression of SOC3. A mutation of the protein that disrupts normal function has been identified as the cause of MODY5 (Maturity-Onset of Diabetes, Type 5) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P35680

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human Hepatocyte nuclear factor 1 homeobox B (HMAQQPFMAAVTQLQN) was used as the immunogen for this HNF1B antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the HNF1B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HNF1B antibody is available for research use only.

Storage Conditions

After reconstitution, the HNF1B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the HNF1B antibody may be required due to differences in protocols and secondary/substrate sensitivity.