PIAS1 Antibody

E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1) ] family. It contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. PIAS1 can also function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL

UniProt

O75925

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 636-651 (DTASIFGIIPDIISLD-human) were used as the immunogen for this PIAS1 antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the PIAS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PIAS1 antibody is available for research use only.

Storage Conditions

After reconstitution, the PIAS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the PIAS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of PIAS1 antibody and Lane 1: rat testis; 2: mouse testis; 3: human HeLa; 4: (h) Jurkat; 5: (h) MCF-7; 6: (h) SKOV lysate; Expected size: 72~100KD