HNF6 Antibody / Hepatocyte nuclear factor 6 / ONCECUT1

One cut homeobox 1 (ONECUT1), also called Hepatocyte nuclear factor 6 (HNF6) is found strong expression in liver and lower expression in testis and skin. The gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q9UBC0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the N-terminus of human ONECUT1/HNF6 (LGSIHNSQQGLPHYAHP) was used as the immunogen for this HNF6 antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the HNF6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HNF6 antibody is available for research use only.

Storage Conditions

After reconstitution, the HNF6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the HNF6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) human HepG2, 2) human HUH7, 3) rat liver, 4) rat RH35 and 5) mouse liver tissue lysate with HNF6 antibody. Predicted molecular weight ~51 kDa.