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KLF4 Antibody

Kruppel-like factor 4, also known as EZF or GKLF, is a protein that in humans is encoded by the KLF4 gene. This gene is mapped to 9q31.2 and encodes a member of the Kruppel family of transcription factors. It plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. KLF4 is required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. The protein is also involved in the differentiation of epithelial cells and may function in skeletal and kidney development.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

O43474

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the N-terminus of human KLF4 (RRETEEFNDLLDLDFI) was used as the immunogen for this KLF4 antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

Ig

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the KLF4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This KLF4 antibody is available for research use only.

Storage Conditions

After reconstitution, the KLF4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the KLF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of KLF4 antibody and Lane 1: HeLa; 2: A549; 3: U20S cell lysate. Predicted molecular weight: 50-60 kDa + possible ~75 kDa (phosphorylated form) .

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