IRF8 Antibody / Interferon regulatory factor 8

Interferon regulatory factor 8, also known as Interferon consensus sequence-binding protein (ICSBP), is a protein that in humans is encoded by the IRF8 gene. By genomic sequence analysis, the gene is mapped to chromosome 16q24.1. It is a transcription factor of the interferon regulatory factor (IRF) family. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFNalpha and IFNbeta. IRF family proteins also control expression of IFNalpha and IFNbeta-regulated genes that are induced by viral infection. IRF8 is a transcription factor that plays critical roles in the regulation of lineage commitment and in myeloid cell maturation including the decision for a common myeloid progenitor (CMP) to differentiate into a monocyte precursor cell.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q02556

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

An amino acid sequence from the C-terminus of human Interferon regulatory factor 8 (ASHQRSFFRENQQITV) was used as the immunogen for this IRF8 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the IRF8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This IRF8 antibody is available for research use only.

Storage Conditions

After reconstitution, the IRF8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the IRF8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunofluorescent staining of FFPE human U-2 OS cells with IRF8 antibody (green) and Alpha Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.