OCT1 Antibody / SLC22A1

Solute carrier family 22 (organic cation transporter) member 1, also called OCT1 (Organic cation transporter 1) is a protein that in humans is encoded by the SLC22A1 gene. This gene is mapped to 6q25.3. Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene transports the polyamines spermine and spermidine. It also transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. This gene regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

O15245

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human SLC22A1/OCT1 (RKAKPKENTIYLKVQTSE) was used as the immunogen for this OCT1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the OCT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This OCT1 antibody is available for research use only.

Storage Conditions

After reconstitution, the OCT1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the OCT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of OCT1 antibody and Lane 1: human HeLa cell lysate; 2: human A549 cell lysate. Predicted molecular weight ~61 kDa but this glycoprotein may be observed at up to ~80 kDa.