MMP12 Antibody

Matrix metalloproteinase-12, also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is though that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P39900

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human Matrix metalloproteinase-12 (KIDAVFYSKNKYYYFFQ) was used as the immunogen for this MMP12 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the MMP12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MMP12 antibody is available for research use only.

Storage Conditions

After reconstitution, the MMP12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the MMP12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of MMP12 antibody and Lane 1: human SMMC-7721; 2: mouse HEPA; 3: (h) HeLa; 4: (h) K562; 5: (h) MCF-7 cell lysate. Predicted molecular weight: ~55 kDa (pro form), ~45 kDa and ~22 kDa (active forms) .