Collagen 2 Antibody / COL2A1

Collagen type 2 alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocytes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P02458

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human COL2A1 (AFAGLGPREKGPDPLQYMRA) was used as the immunogen for this Collagen 2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Collagen Type 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Collagen 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Collagen 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Collagen 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Collagen 2 antibody and human samples 1: COLO320; 2: HeLa; 3: MCF-7; 4: A549; 5: Jurkat lysate. Predicted molecular weight ~140 kDa.