Collagen 1 Antibody

Collagen type 1 alpha 1, also known as COL1A1, is a human gene that encodes the major component of type 1 collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type 1 collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Collagen type 1 is fibril-forming and found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunocytochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL

UniProt

P02452

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of mouse COL1A1 (QPPQEKSQDGGRYYRA) was used as the immunogen for this Collagen 1 antibody (100% rat homology) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Collagen type 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Collagen 1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Collagen 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Collagen 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human placental tissue with Collagen 1 antibody (red) and DAPI counterstain (blue) . HIER: boil tissue sections in pH8 EDTA buffer, for 10-20 min and allow to cool before testing.