PTH1R Antibody

Parathyroid hormone/parathyroid hormone-related peptide receptor, also known as PTHR, is a protein that in humans is encoded by the PTH1R gene. PTHR is a member of a family of G protein-coupled receptors. PTHR gene was mapped to the vicinity of the 3p21.3-p21.2 boundary by PCR analysis of human/rodent somatic cell hybrid panels using oligonucleotide primers designed to amplify a portion of the gene from genomic DNA. This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. PTH1R functions as a receptor for parathyroid hormone and for parathyroid hormone-related protein.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL

UniProt

Q03431

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human Parathyroid Hormone Receptor 1 (KLRETNAGRCDTRQQYRKL) was used as the immunogen for this PTH1R antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the PTH1R antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PTH1R antibody is available for research use only.

Storage Conditions

After reconstitution, the PTH1R antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the PTH1R antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) SK-OV-3, 2) U-2 0S, 3) HeLa and 4) SMMC-7721 cell lysate. Expected molecular weight ~66 kDa (unmodified), 85-95 kDa (glycosylated) .