Cdk7 Antibody

Cyclin-dependent kinase 7, also known as Cell division protein kinase 7, is an enzyme that in humans is encoded by the CDK7 gene. The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. The gene was assigned to human chromosome 5q13.2. Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. It is required for DNA-bound peptides-mediated transcription and cellular growth inhibition.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P50613

Host

Rabbit

Reactivity

Human, Rat, Mouse

Immunogen

An amino acid sequence from the C-terminus of human Cyclin-dependent kinase 7 (RKRTEALEQGGLPKKLIF) was used as the immunogen for this Cdk7 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Cdk7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cdk7 antibody is available for research use only.

Storage Conditions

After reconstitution, the Cdk7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Cdk7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Cdk7 antibody and Lane 1: HeLa; 2: MCF-7; 3: A549; 4: COLO320; 5: Jurkat cell lysate. Expected/observed molecular weight ~37 kDa.