BAG5 Antibody

BAG family molecular chaperone regulator 5 is a protein that in humans is encoded by the BAG5 gene. The protein encoded by this gene is a member of the BAG1-related protein family. BAG5 is a negative regulator of both Hsp70 and parkin function that sensitizes dopaminergic neurons to injury-induced death and thus promotes neurodegeneration.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q9UL15

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human BAG5 (QGEEKCKAARKQAVR) was used as the immunogen for this BAG5 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the BAG5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This BAG5 antibody is available for research use only.

Storage Conditions

After reconstitution, the BAG5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the BAG5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Flow cytometry testing of human A431 cells with BAG5 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= BAG5 antibody.