Ataxin-2 Antibody

Ataxin-2, the protein encoded by the ATXN2 gene, contains a polyglutamine tract, long expansions (greater than 33 repeats) of which result in spinocerebellar ataxia-2 (SCA2), an autosomal dominant form of olivopontocerebellar atrophy. The gene for spinocerebellar ataxia type 2 (SCA2) has been mapped to 12q24.1. Ataxin-2 associates with L- and T-plastin and overexpression leads to accumulation of T-plastin in mammalian cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, FACS: 1-3ug/10^6 cells, Immunofluorescence (FFPE) : 5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q99700

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human Ataxin-2 (TTAHFPYMTHPSVQAHHQQQL) was used as the immunogen for this Ataxin-2 antibody (100% mouse homology) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IF, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Ataxin-2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Ataxin-2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Ataxin-2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Ataxin-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human breast cancer tissue with Ataxin-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.