P63 Antibody

Tumor Protein p63, also known as KET, is a protein that in humans is encoded by the TP63 gene. Yang et al. (1998) described the cloning of tumor protein, which shows strong homology to the tumor suppressor p53 and the p53-related protein p73. By fluorescence in situ hybridization, Yang et al. (1998) localized the human gene to chromosome 3q27-q29. Hibi et al. (2000) stated that p53 homologs known variously as p40, p51, p63, and p73L (Trink etal., 1998, Yang etal., 1998, Osada etal., 1998, Senoo etal., 1998) are isoforms of the same gene, which were then referred to as AIS for 'amplified in squamous cell carcinoma.'

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q9H3D4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 82-98 (DMDARRNKQQRIKEEGE) were used as the immunogen for this p63 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the p63 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This p63 antibody is available for research use only.

Storage Conditions

After reconstitution, the p63 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the p63 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunofluorescent staining of FFPE human A431 cells with p63 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.