RSK1 Antibody

Ribosomal Protein S6 Kinase, 90-KD, 1, also called RSK1, is an enzyme that in humans is encoded by the RPS6KA1 gene. The RSK (ribosomal S6 kinase) family comprises growth factor-regulated serine/threonine kinases, known also as p90 (RSK) . Homologs exist in several species. By analysis of somatic cell hybrids, Moller et al. (1994) mapped the gene to chromosome 3. Bonni et al. (1999) characterized the mechanism by which the RAS-MAPK signaling pathway mediates growth factor-dependent cell survival. Inoue et al. (2007) demonstrated that p90RSK, the kinase immediately downstream from Mos-MAPK, directly targets Erp1 for CSF arrest in Xenopus oocytes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q15418

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human RSK1 (ILAQRRVRKLPSTTL) was used as the immunogen for this RSK1 antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the RSK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This RSK1 antibody is available for research use only.

Storage Conditions

After reconstitution, the RSK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the RSK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

Western blot testing of RSK1 antbody; Lane 1: MCF-7; 2: HeLa; 3: K562; 4: Jurkat; 5: SW620; 6: Raji cell lysate. Predicted molecular weight: 83~90 kDa.