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EIF3B Antibody

Eukaryotic Translation Initiation Factor 3, Subunit B, also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al. (1997) identified a cDNA encoding a human Prt1 homolog. Asano et al. (1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of two proteins, p116 (EIF3B) and an unrelated 110-kD protein.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P55884

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human Eukaryotic Translation Initiation Factor 3, Subunit B (EDFRKYRKMAQELYMEQKNER) was used as the immunogen for this Eif3b antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the EIF3B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This EIF3B antibody is available for research use only.

Storage Conditions

After reconstitution, the EIF3B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Product Datasheet

https://www.nsjbio.com/tds-pdf/eif3b-antibody-r31139

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the EIF3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) A375, 2) K562 and 3) MCF7 cell lysate with EIF3B antibody. Expected molecular weight ~116 kDa.

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