DISC1 Antibody

Disrupted in Schizophrenia 1 is a protein that is encoded by the DISC1 gene in humans. Ma et al. (2002) determined that the mouse gene maps to chromosome 8 in a region with homology of synteny to human chromosome 1q42. Ozeki et al. (2003) demonstrated that rodent Disc1 expression displayed pronounced developmental regulation, with the highest levels in late embryonic life during development of the cerebral cortex. Millar et al. (2005) showed that the protein interacts with the UCR2 domain of phosphodiesterase-4B, implicated in susceptibility to schizophrenia, and that elevation of cellular cAMP leads to dissociation of PDE4B from DISC1 and in increase in PDE4B activity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

Q9NRI5

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

An amino acid sequence from the middle region of human Disrupted in Schizophrenia 1 (EKQQLQKEIEALQARMF) was used as the immunogen for this DISC1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the DISC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This DISC1 antibody is available for research use only.

Storage Conditions

After reconstitution, the DISC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the DISC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.