Adiponectin Antibody

Adiponectin, als called ADIPOQ (Adipocyte-, C1q-, and Collagen Domain-Containing), APM1, ADPN and ACDC, is a protein which in humans is encoded by the ADIPOQ gene. Using FISH, Das et al. (2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, Schaffler et al. (1999) showed that ADIPOQ is expressed by differentiated adipocytes as a 30-kD protein that is also detectable in serum. By sequence comparisons, they found links between ADIPOQ and TNF family ligands as well as to cytokines expressed by T cells. It is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. It is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested)

UniProt

Q15848

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human ADIPOQ (LYADNDNDSTFTGFLLYHDTN) was used as the immunogen for this Adiponectin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F, ELISA (protein)

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide and 0.025% thimerosal

Reconstitution

After reconstitution, the Adiponectin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Adiponectin antibody is available for research use only.

Storage Conditions

After reconstitution, the Adiponectin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Adiponectin antibody may be required due to differences in protocols and secondary/substrate sensitivity.