T-bet Antibody (TBX21)

TBX21 (T-Box 21), also called T-bet (T-box expressed in T cells), is a protein that in humans is encoded by the TBX21 gene. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. Zhang and Yang (2000) mapped the mouse Tbx21 gene to chromosome 11D in an area showing homology of synteny with human chromosome 17. Szabo et al. (2000) showed that expression correlates with IFNG expression in Th1 and natural killer (NK) cells. Ectopic expression of T-bet both transactivated the IFNG gene and induced endogenous IFNG production. Finotto et al. (2002) observed reduced expression of the T (H) 1 transcription factor T-bet in T cells from airways of patients with asthma compared with that in T cells from airways of nonasthmatic patients, suggesting that loss might be associated with asthma.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q9UL17

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human T-box expressed in T cells (DKEAEGQFYNYFPN) was used as the immunogen for this T-bet antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the T-bet antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This T-bet antibody is available for research use only.

Storage Conditions

After reconstitution, the T-bet antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the T-bet antibody may be required due to differences in protocols and secondary/substrate sensitivity.