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SCLY Antibody / Selenocysteine lyase

Selenocysteine lyase, is an enzyme that catalyzes the chemical reaction. This enzyme belongs to the family of lyases, specifically the class of carbon-sulfur lyases. The International Radiation Hybrid Mapping Consortium mapped the human SCLY gene to chromosome 2. Mihara et al. (2000) showed that mouse Scly catalyzed the conversion of L-selenocysteine to L-alanine. Scly activity required pyridoxal 5-prime phosphate, was specific to L-selenocysteine, and showed maximum reactivity at pH 9.0.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q96I15

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human Selenocysteine lyase (QDLKQAVAQLEDQA) was used as the immunogen for this SCLY antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the SCLY antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SCLY antibody is available for research use only.

Storage Conditions

After reconstitution, the SCLY antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the SCLY antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of SCYL antbody on Lane 1: U87; 2: HeLa; 3: 293T; 4: MCF-7; 5: COLO320; 6: HE1080 cell lysate

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