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PDK1 Antibody / Pyruvate dehydrogenase kinase 1

Pyruvate dehydrogenase kinase isoenzyme 1, is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

Q15118

Host

Rabbit

Reactivity

Human, Rat

Immunogen

An amino acid sequence from the C-terminus of human Pyruvate dehydrogenase kinase isoenzyme 1 (WKHYNTNHEADDWC) was used as the immunogen for this PDK1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the PDK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PDK1 antibody is available for research use only.

Storage Conditions

After reconstitution, the PDK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the PDK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of PDK1 antbody on Lane 1: rat heart; 2: (r) skeletal muscle; and human samples 3: HeLa; 4: M231; 5: COLO320; 6: SW620 cell lysate. Predicted molecular weight ~49 kDa, observed here at ~64 kDa.

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