VEGFR2 Antibody

KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR 2 or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Sait et al. (1995) likewise corrected the assignment to chromosome 4q11-q12 to the same region occupied also by PDGFRA and KIT, thus indicating the location of a cluster of receptor tyrosine kinase genes. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells (Plate et al., 1993) . Flk1 is the mouse homolog of KDR (Matthews et al., 1991) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunohistochemistry (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL

UniProt

P35968

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the middle region of human VEGF Receptor 2 (HHIHWYWQLEEECANE) was used as the immunogen for this VEGFR2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the VEGFR2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This VEGFR2 antibody is available for research use only.

Storage Conditions

After reconstitution, the VEGFR2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the VEGFR2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic and cell surface

Image Legend

Western blot testing with VEGFR2 antibody and SMMC-7721 lysate. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature) .