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TJP2 Antibody / ZO-2

Tight Junction Protein 2, also known as Zona Occludens 2 or ZO-2, is a protein that in humans is encoded by the TJP2 gene. Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q9Z0U1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of mouse Tight Junction Protein 2 (AHSKRGYYSQPSRYRDTEL) was used as the immunogen for this TJP2 antibody (100% rat homology) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the TJP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TJP2 antibody is available for research use only.

Storage Conditions

After reconstitution, the TJP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the TJP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC staining of FFPE mouse liver tissue with TJP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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