RIAM Antibody

RIAM (Rap1-interacting adaptor molecule), also called RARP1 and APBB1-Interacting Protein, is a protein that in humans is encoded by the APBB1IP gene. By genomic sequence analysis, Lafuente et al. (2004) mapped the gene to chromosome 10p12.1. Using promoter-reporter gene assays, Inagaki et al. (2003) found that RIAM suppressed transcription from AP1 and SRE sites, but not CRE sites, in all cell lines examined. The proline-rich regions of RIAM suppressed AP1 transactivation. Lafuente et al. (2004) found that the protein interacted with profilin and VASP, molecules that regulate actin dynamics, as well as with RAP1-GTP.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunohistochemistry (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q7Z5R6

Host

Rabbit

Reactivity

Human, Rat

Immunogen

An amino acid sequence from the C-terminus of human Rap1-interacting adaptor molecule (EQDFMSDLMKALQKKRGNVS) was used as the immunogen for this RIAM antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IHC-F, ICC, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the RIAM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This RIAM antibody is available for research use only.

Storage Conditions

After reconstitution, the RIAM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the RIAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of RIAM antibody and human 1) ThP-1, 2) Jurkat, 3) Raji, 4) U937, 5) rat thymus and 6) mouse thymus lysate. Predicted molecular weight ~73~110 kDa.