CD31 Antibody

PECAM-1 (Platelet endothelial cell adhesion molecule), also known as cluster of differentiation 31 (CD31) is a protein that in human is encoded by the PECAM1 gene found on chromosome 17. It is a member of the immunoglobulin (Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and particular T-cell subsets. Using a PCR-based analysis of somatic cell hybrids, Gumina et al. (1996) mapped CD31 to chromosome 17 in the region 17q23-qter. Several adhesion molecules expressed on platelets and endothelium also localized to 17q. Xie and Muller (1996) mapped the Pecam1 gene to mouse chromosome 6, region F3-G1, by fluorescence in situ hybridization. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions, and it plays a key role in removing aged neutrophils from the body.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL

UniProt

P16284

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human CD31 (RKAVPDAVESRYSRTE) was used as the immunogen for this CD31 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CD31 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CD31 antibody is available for research use only.

Storage Conditions

After reconstitution, the CD31 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the CD31 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunofluorescent staining of FFPE human placental tissue with CD31 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.