TFAM Antibody / mtTFA

Transcription factor A, mitochondrial (TFAM), also known as TCF6 or TCF6L2, is a protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to three different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse TFAM to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q00059

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of human Transcription factor A mitochondrial (RKDLLRRTIKKQRKYGAEEC) was used as the immunogen for this TFAM antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the TFAM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TFAM antibody is available for research use only.

Storage Conditions

After reconstitution, the TFAM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the TFAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human liver cancer tissue with TFAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.