Nucleophosmin Antibody / NPM1

Nucleophosmin, also known as NPM, is a protein that in humans is encoded by the NPM1 gene. The gene maps to chromosome 5q35. Nucleophosmin is likely involved in the assembly of ribosomal proteins into ribosomes. An electron microscopic study indicated that Nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P06748

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

An amino acid sequence from the N-terminus of human NPM1 (ELKADKDYHFKVDNDENEHQ) was used as the immunogen for this Nucleophosmin antibody (100% homologous in human, mouse and rat) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

The lyophilized Nucleophosmin antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

Limitations

This Nucleophosmin antibody is available for research use only.

Storage Conditions

The lyophilized Nucleophosmin antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Variations in secondary/substrate sensitivities and test protocols may require the Nucleophosmin antibody to be titrated for optimal performance.

Location

Nucleus, cytoplasm

Image Legend

IHC staining of FFPE human thyroid cancer tissue with Nucleophosmin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.