NOXA1 Antibody / NADPH oxidase activator 1

NADPH oxidase activator 1, also called NOX Activator 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz (2007) mapped the gene to chromosome 9q34.3 based on an alignment of the protein sequence with the genomic sequence (build 36.1) . Banfi et al. (2003) mapped the mouse gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al. (2003) showed that the human protein interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that NOXA1 bound GTP-bound RAC1, but not GDP-bound RAC1. It also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of NOXA1 was required for these interactions. Human cell lines or COS-7 cells cotransfected with NOXA1/p51-NOX and p41-NOX and either gp91-PHOX (CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.