NOX4 Antibody

NADPH oxidase 4, also called Renal NADPH oxidase or RENOX, is an enzyme that in humans is encoded by the NOX4 gene, and a member of the NOX family of NADPH oxidases. Geiszt et al. (2000) stated that the nucleotide sequence of NOX4 matches that found in a genomic clone on chromosome 15. In a note added in proof, they stated that genomic clones assigned to chromosome 11 also contain sequence corresponding to NADPH Oxidase 4. By FISH, Shiose et al. (2001) mapped the gene to chromosome 11q14.2-q21. Geiszt et al. (2000) found that NIH 3T3 fibroblasts overexpressing the transfected protein showed increased production of superoxide and developed signs of cellular senescence. They suggested that NOX4, as a renal source of ROS, may fulfill the function of the putative oxygen sensor in the kidney. By biochemical analysis of endogenous renal NOX4, Shiose et al. (2001) determined that the enzyme can use either NADH or NADPH as an electron donor for superoxide production.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q9JHI8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of mouse NADPH oxidase 4 (NRNNSYGTKFEYNKES) was used as the immunogen for this NOX4 antibody (100% rat homology) .

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the NOX4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NOX4 antibody is available for research use only.

Storage Conditions

After reconstitution, the NOX4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the NOX4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.