Smad2 Antibody

SMAD family member 2, also known as MADR2, and MADH2 (Mothers against decapentaplegic homolog 2), is a protein that in humans is encoded by the SMAD2 gene. It belongs to the SMAD family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene 'Sma.' The gene has been mapped to 18q21.1, a region which is frequently deleted in colorectal cancers. SMAD2 mediates the signal of the transforming growth factor (TGF) -beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL

UniProt

Q15796

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the N-terminus of human Smad2 (DQWDTTGLYSFSEQTRSLD) was used as the immunogen for this Smad2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Smad2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Smad2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Smad2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Smad2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.