Smad2 Antibody
SMAD family member 2, also known as MADR2, and MADH2 (Mothers against decapentaplegic homolog 2), is a protein that in humans is encoded by the SMAD2 gene. It belongs to the SMAD family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene 'Sma.' The gene has been mapped to 18q21.1, a region which is frequently deleted in colorectal cancers. SMAD2 mediates the signal of the transforming growth factor (TGF) -beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL
UniProt
Q15796
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the N-terminus of human Smad2 (DQWDTTGLYSFSEQTRSLD) was used as the immunogen for this Smad2 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC-P, ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
Limitations
This Smad2 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Image Legend
IHC-P: Smad2 antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.
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