Ibsp Antibody / Bone sialoprotein 2

Integrin-binding sialoprotein, also known as Bone sialoprotein and BSP, is a major structural protein of the bone matrix. Bone sialoprotein is a predicted 35KD acidic glycoprotein that undergoes extensive posttranslational modifications and is visualized by western blot at up to 70KD. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL

UniProt

Q61711

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of mouse Bone sialoprotein (SYYKGHGYEGYEGQNYYYHQ) was used as the immunogen for this Ibsp antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the IBSP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Ibsp antibody is available for research use only.

Storage Conditions

After reconstitution, the Ibsp antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Ibsp antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Secreted

Image Legend

IHC staining of FFPE mouse pancreas tissue with Ibsp antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.