CD163 Antibody

Cluster of Differentiation 163, also known as Hemoglobin scavenger receptor, is a human protein encoded by the CD163 gene. The receptor belongs to the scavenger receptor cysteine rich family type B and consists of an 1048 amino acid residues extracellular domain, a single transmembrane segment and a cytoplasmic tail with several splice variants. CD163 is a scavenger receptorfor the hemoglobin-haptoglobin complex. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, Stover et al. (2000) mapped the gene to chromosome 12p13.3. CD163 expression in monocytes promoted bacteria-induced proinflammatory cytokine production that could be blocked by CD163 antibody. Cells expressing human CD163 and recombinant protein containing the extracellular domain of CD163 supported adhesion of erythroblastic cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q86VB7

Host

Rabbit

Reactivity

Human

Immunogen

A human partial partial recombinant protein (amino acids 1056-1165) was used as the immunogen for this CD163 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CD163 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CD163 antibody is available for research use only.

Storage Conditions

After reconstitution, the CD163 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the CD163 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface, cytoplasmic

Image Legend

IHC staining of FFPE human lung cancer tissue with CD163 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.