ABCG2 Antibody (BCRP)

ABCG2 (ATP-binding cassette, subfamily G, member 2) also known as ABCP, BCRP (Breast cancer resistance protein) or MRX (Mitoxantrone resistance-associated protein), is a protein that in humans is encoded by the ABCG2 gene. The gene encodes a membrane transporter belonging to the ATP-binding cassette (ABC) superfamily of membrane transporters, which are involved in the trafficking of biologic molecules across cell membranes.The protein is also a high capacity transporter for uric acid excretion in the kidney, liver, and gut. In vitro assays of isolated membrane preparations revealed a high-capacity, vanadate-sensitive ATPase activity associated with ABCG2 expression that was stimulated by compounds known to be transported by this protein.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q7TMS5

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids 152-167 (KNHEKNERINTIIKEL-mouse) were used as the immunogen for this BCRP / ABCG2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

The lyophilized ABCG2 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ABCG2 antibody is available for research use only.

Storage Conditions

The lyophilized ABCG2 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the ABCG2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of ABCG2 antibody and Lane 1: rat liver; 2: rat spleen; 3: rat kidney; 4: mouse spleen tissue lysate. Predicted molecular weight ~72kDa.