SP4 Antibody

Transcription factor Sp4, also known as SPR-1, and HF1B, is a protein that in humans is encoded by the SP4 gene. It is a multigene family of SP proteins. Northern blot analysis shows that it is abundantly expressed as a 7.5-kb message in brain, but barely detectable in other human cell lines. It is an activator protein like SP1. However, in contrast to SP1, Sp4 is not able to act synergistically through adjacent binding sites.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

Q02446

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the N-terminus of human Transcription factor Sp4 (ENNNKKPKTSGSQDSQ) was used as the immunogen for this SP4 antibody (100% rat homology) .

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Sp4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SP4 antibody is available for research use only.

Storage Conditions

After reconstitution, the SP4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Transcription factor SP4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

Western blot testing of SP4 antibody and Lane 1: rat spleen; and human samples 2: SGC; 3: HeLa; 4: Jurkat; 5: A549 cell lysate. Predicted/observed molecular weight ~82kDa.