NME2 Antibody / NM23-H2

NME2 (NME/NM23 nucleoside diphosphate kinase 2) also called non-metastatic cells 2, protein (NM23B) expressed in, NM23-H2, NM23B or NDPKB, is identical to the beta subunit of human erythrocyte NDP kinase. Using Northern blot analysis, Masse et al. (2002) detected high expression of mouse NME2, which they called nm23-M2, in heart, liver, and kidney, with intermediate expression in skeletal muscle. In situ hybridization of 15-day postcoitum mouse embryos showed ubiquitous expression. The mouse and human genes contain 5 exons and span about 6.0 kb. The NME2 gene is mapped on 17q21.33. Srivastava et al. (2006) concluded that histidine phosphorylation regulates KCa3.1 channel activity and that NME2 is critical to the channel activity and the activation of CD4 T cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P22392

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 137-152 (EELVDYKSCAHDWVYE-human) were used as the immunogen for this NME2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the NME2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NME2 antibody is available for research use only.

Storage Conditions

After reconstitution, the NME2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the NME2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IF/ICC staining of FFPE human U-2 OS cells with GNB1 antibody (red) at 2ug/ml and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.