NM23 Antibody

NME1 (NME/NM23 nucleoside diphosphate kinase 1), also called non-Metastatic protein 23 (NM23), is an enzyme that in humans encoded by the NME1 gene. The promoters of the mouse and human NME1 genes, like those of other NME genes, contain several binding sites for AP2, NF1, Sp1, LEF1, and response elements to glucocorticoid receptors. Immunofluorescence microscopy demonstrated colocalization of NME1 in nuclei of B cells expressing EBNA3C. Expression of EBNA3C reversed the ability of NME1 to inhibit migration of BL and breast carcinoma cells. NME1/NM23 bound SET and was released from inhibition by GZMA cleavage of SET. After GZMA loading or cytotoxic T lymphocyte attack, SET and NM23 translocated to the nucleus and SET was degraded, allowing NM23 to nick chromosomal DNA. Using a Drosophila model system, Dammai et al. (2003) showed that the Drosophila NME1 homolog, AWD, regulates trachea cell motility by modulating FGFR levels through a dynamin -mediated pathway.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, FACS: 1-3ug/10^6 cells

UniProt

P15531

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of human NM23 (EELVDYTSCAQNWIYE) was used as the immunogen for this NM23 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the NM23 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NM23 antibody is available for research use only.

Storage Conditions

After reconstitution, the NM23 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the NM23 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of rat lysate samples: 1) heart, 2) brain, 3) liver, 4) skeletal muscle and human lysate samples: 5) PANC-1, 6) HeLa, 7) SK-OV-3 and 8) COLO320. Predicted molecular weight ~17 kDa.